{"id":57720,"date":"2000-03-01T00:00:00","date_gmt":"2018-12-04T06:43:34","guid":{"rendered":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/effects-of-beta-carotene-on-antioxidant-enzyme-activity\/"},"modified":"2018-12-04T07:43:34","modified_gmt":"2018-12-04T06:43:34","slug":"effects-of-beta-carotene-on-antioxidant-enzyme-activity","status":"publish","type":"resume","link":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/effects-of-beta-carotene-on-antioxidant-enzyme-activity\/","title":{"rendered":"Effects of beta-carotene on antioxidant enzyme activity, intracellular reactive oxygen and membrane integrity within post confluent Caco-2 intestinal cells"},"content":{"rendered":"<p>As encountered with a plethora of other natural products, the antioxidant activity of beta-carotene has been proposed as one of the mechanisms by which diets rich in this pro-vitamin A active carotenoid apparently afford chemoprevention.<\/p>\n<p>Here, we report the ability of beta-carotene to alter endogenous reactive oxygen levels and antioxidant defences within non-stressed &#039;differentiated&#039; monolayers of an intestinal epithelial cell line (Caco-2) and to subsequently effect resistance to general oxidative insult.<\/p>\n<p>The differentiated monolayers efficiently absorbed beta-carotene. Between 3 and 8 days post confluence, cultures exhibited a progressive increase in antioxidant enzyme activity and a corresponding reduction to intracellular ROS levels. The profile for antioxidant enzyme activity was unaffected by sustained daily supplementation with beta-carotene. However, after two daily treatments with 50 mu M beta-carotene intracellular ROS levels were significantly reduced and there was a trend towards reduced intracellular ROS within monolayers subject to five daily treatments with 0.5 and 5 mu M beta-carotene. Prolonged supplementation with 0.1 and 0.5 mu M beta-carotene or short supplementation periods with 5 and 50 mu M beta-carotene did not alter susceptibility to H2O2. However, cultures treated daily between 3 and 8 days post confluence with 5 or 50 mu M beta-carotene exhibited enhanced LDH release, increased non-adherence and enhanced Trypan blue staining when challenged with 10 mM H2O2. In the absence of H2O2, the beta-carotene treatments were not overtly toxic to the monolayers.<\/p>\n<p>These results indicate that beta-carotene does not enhance antioxidant defences within Caco-2 monolayers. The enhancement of H2O2 toxicity by persistent, high doses of beta-carotene may contribute to the failure of this carotenoid to protect high risk individuals from certain degenerative conditions.<\/p>\n","protected":false},"template":"","mots_cles":[],"class_list":["post-57720","resume","type-resume","status-publish","hentry"],"acf":{"adresse":"\"BESTWICK CS,ROWETT RES INST,CELL INTEGR PROGRAMME ANTIOXIDANT & FREE RAD GRP;ABERDEEN AB21 9SB, SCOTLAND.csb@rri.sari.ac.uk\"","annee":"2000","mois":"3","numero":"1474:1","page":"47-55","auteurs":[{"ID":9524,"post_author":"0","post_date":"2018-12-03 18:12:04","post_date_gmt":"2018-12-03 17:12:04","post_content":"","post_title":"Charles S Bestwick","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"charles-s-bestwick","to_ping":"","pinged":"","post_modified":"2018-12-03 18:12:04","post_modified_gmt":"2018-12-03 17:12:04","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/charles-s-bestwick\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":16316,"post_author":"0","post_date":"2018-12-03 19:37:17","post_date_gmt":"2018-12-03 18:37:17","post_content":"","post_title":"Ginger L Milne","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"ginger-l-milne","to_ping":"","pinged":"","post_modified":"2018-12-03 19:37:17","post_modified_gmt":"2018-12-03 18:37:17","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/ginger-l-milne\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"}],"sources":[{"ID":49973,"post_author":"0","post_date":"2018-12-04 05:07:18","post_date_gmt":"2018-12-04 04:07:18","post_content":"","post_title":"BIOCHIMICA BIOPHYSICA ACTA","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"biochimica-biophysica-acta","to_ping":"","pinged":"","post_modified":"2018-12-04 05:07:18","post_modified_gmt":"2018-12-04 04:07:18","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/source\/biochimica-biophysica-acta\/","menu_order":0,"post_type":"source","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v23.6 (Yoast SEO v23.6) - 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