{"id":57399,"date":"2000-03-01T00:00:00","date_gmt":"2018-12-04T06:38:48","guid":{"rendered":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/use-of-a-c-tracer-to-quantify-the-plasma-appearance-of-a-physiological\/"},"modified":"2018-12-04T07:38:48","modified_gmt":"2018-12-04T06:38:48","slug":"use-of-a-c-tracer-to-quantify-the-plasma-appearance-of-a-physiological","status":"publish","type":"resume","link":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/use-of-a-c-tracer-to-quantify-the-plasma-appearance-of-a-physiological\/","title":{"rendered":"Use of a C tracer to quantify the plasma appearance of a physiological dose of lutein in humans"},"content":{"rendered":"<p>Increased intake of lutein from vegetables promotes increased density of the macular pigment and therefore may protect against age-related macular degeneration.<\/p>\n<p>Our objective was to use a C-tracer and high-precision gas chromatography-combustion interfaced-isotope ratio mass spectrometry (GC-C-IRMS) to investigate metabolism of lutein dose equivalent to that absorbed from vegetables.<\/p>\n<p>Biosynthetic per-labeled (&gt;99% C) lutein was purified from a commercially available extract of of algal biomass. Subjects (n=4) ingested 3 mg of [C] lutein with a standardized low-carotenoid breakfast. Blood samples were collected at baseline and then hourly for 12 h ; additional blood samples were drawn at 16, 24, 48, 72, 96, 192, 360, and 528 h. To produce parhydro-beta-carotene suitable for analysis by GC-C-IRMS, the plasma lutein fraction was hydrogenated on palladium-on-carbon catalyst with acid-catalyzed hydrogenolysis. The stable carbon isotope (C\/C) ratio mesasured by GC-C-IRMS was used to calculate the plasma concentration of [C] lutein. There was a rapid increase in [C] lutein in plasma until peak enrichment at 16 h followed by by a decline to the next measurement at 24 h. At 528 h, small changes in C enrichment from baseline could still be measured in plasma lutein. High-precision GC-C-IRMS enables complete definition of the appearance and disappearance of [C] lutein in plasma after ingestion of a dose similar to that absorbed from foods.<\/p>\n","protected":false},"template":"","mots_cles":[],"class_list":["post-57399","resume","type-resume","status-publish","hentry"],"acf":{"adresse":"Department of food science and human nutrition, center for designing foods to improve nutrition, and department of chemistry, iowa state university, ames, iowa 50011","annee":"2000","mois":"3","numero":"35:3","page":"339-348","auteurs":[{"ID":27608,"post_author":"0","post_date":"2018-12-03 22:55:17","post_date_gmt":"2018-12-03 21:55:17","post_content":"","post_title":"Yaqiong Liang","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"yaqiong-liang","to_ping":"","pinged":"","post_modified":"2018-12-03 22:55:17","post_modified_gmt":"2018-12-03 21:55:17","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/yaqiong-liang\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":57397,"post_author":"0","post_date":"2018-12-04 07:38:47","post_date_gmt":"2018-12-04 06:38:47","post_content":"","post_title":"Serfass","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"serfass","to_ping":"","pinged":"","post_modified":"2018-12-04 07:38:47","post_modified_gmt":"2018-12-04 06:38:47","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/serfass\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":57398,"post_author":"0","post_date":"2018-12-04 07:38:47","post_date_gmt":"2018-12-04 06:38:47","post_content":"","post_title":"Trahavovsky","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"trahavovsky","to_ping":"","pinged":"","post_modified":"2018-12-04 07:38:47","post_modified_gmt":"2018-12-04 06:38:47","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/trahavovsky\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":51808,"post_author":"0","post_date":"2018-12-04 05:44:44","post_date_gmt":"2018-12-04 04:44:44","post_content":"","post_title":"White","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"white-8","to_ping":"","pinged":"","post_modified":"2018-12-04 05:44:44","post_modified_gmt":"2018-12-04 04:44:44","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/white-8\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50744,"post_author":"0","post_date":"2018-12-04 05:22:58","post_date_gmt":"2018-12-04 04:22:58","post_content":"","post_title":"Yao","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"yao","to_ping":"","pinged":"","post_modified":"2018-12-04 05:22:58","post_modified_gmt":"2018-12-04 04:22:58","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/yao\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"}],"sources":[{"ID":40190,"post_author":"0","post_date":"2018-12-04 02:10:22","post_date_gmt":"2018-12-04 01:10:22","post_content":"","post_title":"LIPIDS","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"lipids","to_ping":"","pinged":"","post_modified":"2018-12-04 02:10:22","post_modified_gmt":"2018-12-04 01:10:22","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/source\/lipids\/","menu_order":0,"post_type":"source","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v23.6 (Yoast SEO v23.6) - 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