{"id":56639,"date":"2001-03-01T00:00:00","date_gmt":"2018-12-04T06:26:30","guid":{"rendered":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/induction-of-apoptosis-by-garcinol-and-curcumin-through-cytochrome\/"},"modified":"2018-12-04T07:26:30","modified_gmt":"2018-12-04T06:26:30","slug":"induction-of-apoptosis-by-garcinol-and-curcumin-through-cytochrome","status":"publish","type":"resume","link":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/induction-of-apoptosis-by-garcinol-and-curcumin-through-cytochrome\/","title":{"rendered":"Induction of apoptosis by garcinol and curcumin through cytochrome c release and activation of caspases in human leukemia HL-60 cells"},"content":{"rendered":"<p>Garcinol, a polyisoprenylated benzophenone, was purified from Garcinia indica fruit rind. The effects of garcinol and curcumin on cell. viability in human leukemia HL-60 cells were investigated. Garcinol and curcumin displayed strong growth inhibitory effects against human leukemia HL-60 cells, with estimated IC50 values of 9.42 and 19.5 &micro;M, respectively. Garcinol was able to induce apoptosis in a concentration- and time-dependent manner;however, curcumin was less effective. Treatment with garcinol caused induction of caspase-3\/CPP32 activity in a dose- and time-dependent manner, but not caspase-1 activity, and induced the degradation of poly(ADP-ribose) polymerase (PARP). Pretreatment with caspase-3 inhibitor inhibited garcinol-induced DNA fragmentation. Treatment with garcinol (20 &micro;M) caused a rapid loss of mitochondrial transmembrane potential, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 processing. The cleavage of D4-GDI, an abundant hematopoietic cell GDP dissociation inhibitor for the Ras-related Rho family GTPases, occurred simultaneously with the activation of caspase-3 but preceded DNA fragmentation and the morphological changes associated with apoptotic cell death. Of these, Bcl-2, Bad, and Bar were studied. The level of expression of Bcl-2 slightly decreased, while the levels of Bad and Bar were dramatically increased in cells treated with garcinol. These results indicate that garcinol allows caspase-activated deoxyribonuclease to enter the nucleus and degrade chromosomal DNA and induces DFF-45 (DNA fragmentation factor) degradation. It is suggested that garcinol-induced apoptosis is triggered by the release of cytochrome c into the cytosol, procaspase-9 processing, activation of caspase-3 and caspase-2, degradation of PARP, and DNA fragmentation caused by the caspase-activated deoxyribonuclease through the digestion of DFF-45. The induction of apoptosis by garcinol may provide a pivotal mechanism for its cancer chemopreventive action.<\/p>\n","protected":false},"template":"","mots_cles":[],"class_list":["post-56639","resume","type-resume","status-publish","hentry"],"acf":{"adresse":"\"LIN JK,NATL TAIWAN UNIV,COLL MED INST BIOCHEM;1 SECT 1,JEN AI RD; TAIWAN.jklin@ha.mc.ntu.edu.tw\"","annee":"2001","mois":"3","numero":"49:3","page":"1464-1474","auteurs":[{"ID":56637,"post_author":"0","post_date":"2018-12-04 07:26:28","post_date_gmt":"2018-12-04 06:26:28","post_content":"","post_title":"Chang","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"chang-15","to_ping":"","pinged":"","post_modified":"2018-12-04 07:26:28","post_modified_gmt":"2018-12-04 06:26:28","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/chang-15\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":9120,"post_author":"0","post_date":"2018-12-03 18:07:36","post_date_gmt":"2018-12-03 17:07:36","post_content":"","post_title":"Chi-Tang Ho","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"chi-tang-ho","to_ping":"","pinged":"","post_modified":"2018-12-03 18:07:36","post_modified_gmt":"2018-12-03 17:07:36","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/chi-tang-ho\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":48372,"post_author":"0","post_date":"2018-12-04 04:32:43","post_date_gmt":"2018-12-04 03:32:43","post_content":"","post_title":"Collins","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"collins-4","to_ping":"","pinged":"","post_modified":"2018-12-04 04:32:43","post_modified_gmt":"2018-12-04 03:32:43","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/collins-4\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":56638,"post_author":"0","post_date":"2018-12-04 07:26:29","post_date_gmt":"2018-12-04 06:26:29","post_content":"","post_title":"Lin-shiau","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"lin-shiau","to_ping":"","pinged":"","post_modified":"2018-12-04 07:26:29","post_modified_gmt":"2018-12-04 06:26:29","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/lin-shiau\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":42730,"post_author":"0","post_date":"2018-12-04 02:54:58","post_date_gmt":"2018-12-04 01:54:58","post_content":"","post_title":"Pan","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"pan","to_ping":"","pinged":"","post_modified":"2018-12-04 02:54:58","post_modified_gmt":"2018-12-04 01:54:58","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/pan\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"}],"sources":[{"ID":1648,"post_author":"0","post_date":"2018-12-03 16:01:16","post_date_gmt":"2018-12-03 15:01:16","post_content":"","post_title":"Journal of agricultural and food chemistry","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"journal-of-agricultural-and-food-chemistry","to_ping":"","pinged":"","post_modified":"2018-12-03 16:01:16","post_modified_gmt":"2018-12-03 15:01:16","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/source\/journal-of-agricultural-and-food-chemistry\/","menu_order":0,"post_type":"source","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v23.6 (Yoast SEO v23.6) - 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