{"id":54130,"date":"2003-06-01T00:00:00","date_gmt":"2018-12-04T05:32:43","guid":{"rendered":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/analysis-of-isothiocyanate-mercapturic-acids-in-urine-a-biomarker\/"},"modified":"2018-12-04T06:32:43","modified_gmt":"2018-12-04T05:32:43","slug":"analysis-of-isothiocyanate-mercapturic-acids-in-urine-a-biomarker","status":"publish","type":"resume","link":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/analysis-of-isothiocyanate-mercapturic-acids-in-urine-a-biomarker\/","title":{"rendered":"Analysis of isothiocyanate mercapturic acids in urine: a biomarker for cruciferous vegetable intake."},"content":{"rendered":"

Cruciferous vegetables contain glucosinolates, which are degraded to isothiocyanates. These are easily absorbed, conjugated to glutathione, and excreted into the urine as their corresponding mercapturic acids. We have developed and validated a solid phase extraction-high-performance liquid chromatography-electrospray ionization mass spectrometry\/mass spectrometry method for the specific analysis of individual isothiocyanate mercapturic acids in urine. The range of reliable analysis was 1.0-310 microM in urine. Urine samples fortified with three different levels of isothiocyanate mercapturic acids were measured on six different days by three independent technicians. The relative standard deviation (RSD) of repeatability was 12, 6, and 3%; the RSD of reproducibility was 19, 14, and 8%, and spike recoveries were 103, 104, and 103%, respectively, for 1.04, 10.5, and 313 microM levels. In 24 h urine collected from two volunteers after they consumed broccoli and cauliflower, clearly sulforaphane mercapturic acid (133 micromol) and allyl isothiocyanate mercapturic acid (4.7 micromol) were found. This procedure demonstrates a reliable and efficient method to study the intake and mode of action of isothiocyanates in animal studies and clinical trials.<\/p>\n","protected":false},"template":"","mots_cles":[],"class_list":["post-54130","resume","type-resume","status-publish","hentry"],"acf":{"adresse":"Department of Food & Food Supplement Analysis, TNO Nutrition and Food Research, P.O. Box 360, 3700 AJ Zeist, The Netherlands. m.vermeulen@voeding.tno.nl","annee":"2003","mois":"6","numero":"51:12","page":"3554-3559","auteurs":[{"ID":48423,"post_author":"0","post_date":"2018-12-04 04:33:39","post_date_gmt":"2018-12-04 03:33:39","post_content":"","post_title":"Vaes","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"vaes","to_ping":"","pinged":"","post_modified":"2018-12-04 04:33:39","post_modified_gmt":"2018-12-04 03:33:39","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/vaes\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":54129,"post_author":"0","post_date":"2018-12-04 06:32:41","post_date_gmt":"2018-12-04 05:32:41","post_content":"","post_title":"Van Rooijen","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"van-rooijen","to_ping":"","pinged":"","post_modified":"2018-12-04 06:32:41","post_modified_gmt":"2018-12-04 05:32:41","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/van-rooijen\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":48425,"post_author":"0","post_date":"2018-12-04 04:33:41","post_date_gmt":"2018-12-04 03:33:41","post_content":"","post_title":"Vermeulen","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"vermeulen","to_ping":"","pinged":"","post_modified":"2018-12-04 04:33:41","post_modified_gmt":"2018-12-04 03:33:41","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/vermeulen\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"}],"sources":[{"ID":1648,"post_author":"0","post_date":"2018-12-03 16:01:16","post_date_gmt":"2018-12-03 15:01:16","post_content":"","post_title":"Journal of agricultural and food chemistry","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"journal-of-agricultural-and-food-chemistry","to_ping":"","pinged":"","post_modified":"2018-12-03 16:01:16","post_modified_gmt":"2018-12-03 15:01:16","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/source\/journal-of-agricultural-and-food-chemistry\/","menu_order":0,"post_type":"source","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"\nAnalysis of isothiocyanate mercapturic acids in urine: a biomarker for cruciferous vegetable intake. - Aprifel<\/title>\n<meta name=\"description\" content=\"Analysis of isothiocyanate mercapturic acids in urine: a biomarker for cruciferous vegetable intake. - Aprifel\" \/>\n<meta name=\"robots\" content=\"noindex, follow\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Analysis of isothiocyanate mercapturic acids in urine: a biomarker for cruciferous vegetable intake.\" \/>\n<meta property=\"og:description\" content=\"Analysis of isothiocyanate mercapturic acids in urine: a biomarker for cruciferous vegetable intake.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/analysis-of-isothiocyanate-mercapturic-acids-in-urine-a-biomarker\/\" \/>\n<meta property=\"og:site_name\" content=\"Aprifel\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/analysis-of-isothiocyanate-mercapturic-acids-in-urine-a-biomarker\/\",\"url\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/analysis-of-isothiocyanate-mercapturic-acids-in-urine-a-biomarker\/\",\"name\":\"Analysis of isothiocyanate mercapturic acids in urine: a biomarker for cruciferous vegetable intake. - 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