{"id":54073,"date":"2003-07-01T00:00:00","date_gmt":"2018-12-04T05:30:44","guid":{"rendered":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/protective-effects-of-cyanidin-3-o-glucoside-from-blackberry\/"},"modified":"2018-12-04T06:30:44","modified_gmt":"2018-12-04T05:30:44","slug":"protective-effects-of-cyanidin-3-o-glucoside-from-blackberry","status":"publish","type":"resume","link":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/protective-effects-of-cyanidin-3-o-glucoside-from-blackberry\/","title":{"rendered":"Protective effects of cyanidin-3-O-glucoside from blackberry extract against peroxynitrite-induced endothelial dysfunction and vascular failure"},"content":{"rendered":"<p style=\"text-align: justify\">Anthocyanins are a group of naturally occurring phenolic compounds as colorants in several plants, flowers and fruits. These pigments have a great importance as quality indicators, as chemotaxonomic markers and antioxidants.The content of blackberry (Rubus species) juice was investigated by HPLC\/ESI\/MS using narrow bore HPLC columns. Using this method we demonstrated that cyanidin-3-O-glucoside represents about 80% of the total anthocyanin contents in blackberry extract. Here we investigated antioxidant activity of the blackberry juice and cyanidin-3-O-glucoside on the endothelial dysfunction in cells and in vascular rings exposed to peroxynitrite. In human umbilical vein endothelial cells (HUVEC) in vitro, peroxynitrite caused a significant suppression of mitochondrial respiration (38 +\/- 2.1% of control cells), as measured by the mitochondrial-dependent conversion of the dye MTT to formazan. Peroxynitrite caused DNA strand breakage (63 +\/- 1.9% single strand vs 3 +\/- 0.9% single strand in control cells), as measured by the alkaline unwinding assay, and caused an activation of PARS, as measured by the incorporation of radiolabeled NAD(+) to nuclear proteins. Blackberry juice (different dilutions that contained 80 ppm;40 ppm;14.5 ppm of cyanidin-3-O-glucoside) and cyanidin-3-O-glucoside (as chloride) (0.085 &micro;M; 0.028 microM; 0.0085 &micro;M) reduced the peroxynitrite-induced suppression of mitochondrial respiration, DNA damage and PARS activation in HUVECs. Vascular rings exposed to peroxynitrite exhibited reduced endothelium-dependent relaxant responses in response to acetylcholine as well as a vascular contractility dysfunction in response to norepinephrine. The development of this peroxynitrite-induced vascular dysfunction was ameliorated by the blackberry juice (different dilutions that contained 80 ppm;40 ppm;14.5 ppm of cyanidin-3-O-glucoside) and cyanidin-3-O-glucoside (as chloride) (0.085 &micro;M;0.028 &micro;M;0.0085 &micro;M).In conclusion our findings clearly demonstrate that blackberry juice containing cyanidin-3-O-glucoside is a scavenger of peroxynitrite and that exert a protective effect against endothelial dysfunction and vascular failure induced by peroxynitrite.<\/p>\n","protected":false},"template":"","mots_cles":[],"class_list":["post-54073","resume","type-resume","status-publish","hentry"],"acf":{"adresse":"Dipartimento Clinico e Sperimentale di Medicina e Farmacologia, Torre Biologica, Policlinico Universitario, 98123 Messina, Italy.","annee":"2003","mois":"7","numero":"73:9","page":"1097-1114","auteurs":[{"ID":53863,"post_author":"0","post_date":"2018-12-04 06:26:46","post_date_gmt":"2018-12-04 05:26:46","post_content":"","post_title":"Caputi","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"caputi-2","to_ping":"","pinged":"","post_modified":"2018-12-04 06:26:46","post_modified_gmt":"2018-12-04 05:26:46","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/caputi-2\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":53864,"post_author":"0","post_date":"2018-12-04 06:26:46","post_date_gmt":"2018-12-04 05:26:46","post_content":"","post_title":"Cuzzocrea","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"cuzzocrea","to_ping":"","pinged":"","post_modified":"2018-12-04 06:26:46","post_modified_gmt":"2018-12-04 05:26:46","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/cuzzocrea\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":6828,"post_author":"0","post_date":"2018-12-03 17:42:24","post_date_gmt":"2018-12-03 16:42:24","post_content":"","post_title":"Giacomo Dugo","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"giacomo-dugo","to_ping":"","pinged":"","post_modified":"2018-12-03 17:42:24","post_modified_gmt":"2018-12-03 16:42:24","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/giacomo-dugo\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":54071,"post_author":"0","post_date":"2018-12-04 06:30:39","post_date_gmt":"2018-12-04 05:30:39","post_content":"","post_title":"Dugo","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"dugo-3","to_ping":"","pinged":"","post_modified":"2018-12-04 06:30:39","post_modified_gmt":"2018-12-04 05:30:39","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/dugo-3\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":53865,"post_author":"0","post_date":"2018-12-04 06:26:47","post_date_gmt":"2018-12-04 05:26:47","post_content":"","post_title":"Dugo","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"dugo-2","to_ping":"","pinged":"","post_modified":"2018-12-04 06:26:47","post_modified_gmt":"2018-12-04 05:26:47","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/dugo-2\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":54072,"post_author":"0","post_date":"2018-12-04 06:30:42","post_date_gmt":"2018-12-04 05:30:42","post_content":"","post_title":"Mazzon","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"mazzon","to_ping":"","pinged":"","post_modified":"2018-12-04 06:30:42","post_modified_gmt":"2018-12-04 05:30:42","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/mazzon\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":53866,"post_author":"0","post_date":"2018-12-04 06:26:48","post_date_gmt":"2018-12-04 05:26:48","post_content":"","post_title":"Mondello","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"mondello-2","to_ping":"","pinged":"","post_modified":"2018-12-04 06:26:48","post_modified_gmt":"2018-12-04 05:26:48","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/mondello-2\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":53867,"post_author":"0","post_date":"2018-12-04 06:26:48","post_date_gmt":"2018-12-04 05:26:48","post_content":"","post_title":"Serraino","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"serraino","to_ping":"","pinged":"","post_modified":"2018-12-04 06:26:48","post_modified_gmt":"2018-12-04 05:26:48","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/serraino\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"}],"sources":[{"ID":4283,"post_author":"0","post_date":"2018-12-03 17:14:11","post_date_gmt":"2018-12-03 16:14:11","post_content":"","post_title":"LIFE SCIENCES","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"life-sciences","to_ping":"","pinged":"","post_modified":"2018-12-03 17:14:11","post_modified_gmt":"2018-12-03 16:14:11","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/source\/life-sciences\/","menu_order":0,"post_type":"source","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v23.6 (Yoast SEO v23.6) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ 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