{"id":50167,"date":"2005-07-01T00:00:00","date_gmt":"2018-12-04T04:12:02","guid":{"rendered":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/"},"modified":"2018-12-04T05:12:02","modified_gmt":"2018-12-04T04:12:02","slug":"fruit-specific-rnai-mediated-suppression-of-det1-enhances","status":"publish","type":"resume","link":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/","title":{"rendered":"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes"},"content":{"rendered":"<p>Abstract: Tomatoes are a principal dietary source of carotenoids and flavonoids, both of which are highly beneficial for human health(1,2). Overexpression of genes encoding biosynthetic enzymes or transcription factors have resulted in tomatoes with improved carotenoid or flavonoid content, but never with both(3-7). We attempted to increase tomato fruit nutritional value by suppressing an endogenous photomorphogenesis regulatory gene, DET1, using fruit-specific promoters combined with RNA interference (RNAi) technology. Molecular analysis indicated that DET1 transcripts were indeed specifically degraded in transgenic fruits. Both carotenoid and flavonoid contents were increased significantly, whereas other parameters of fruit quality were largely unchanged. These results demonstrate that manipulation of a plant regulatory gene can simultaneously influence the production of several phytonutrients generated from independent biosynthetic pathways, and provide a novel example of the use of organ-specific gene silencing to improve the nutritional value of plant-derived products. <\/p>\n","protected":false},"template":"","mots_cles":[],"class_list":["post-50167","resume","type-resume","status-publish","hentry"],"acf":{"adresse":"Addresses: Bowler C (reprint author), Stn Zool, Cell Signaling Lab, Villa Comunale, I-80121 Naples Italy\r\nStn Zool, Cell Signaling Lab, I-80121 Naples Italy\r\nUniv London, Royal Holloway & Bedford New Coll, Egham TW20 0EX Surrey England\r\nDNA Plant Technol Corp, Oakland, CA 94608 USA\r\nSeminis Vegetable Seeds Inc, Woodland, CA 95695 USA\r\nEcole Normale Super, CNRS, ENS, FRE2910, F-75230 Paris France \r\nE-mail Addresses: cbowler@biologie.ens.fr \r\n\r\n\r\n\r\n\r\n\r\n","annee":"2005","mois":"7","numero":"23:7","page":"890-895","auteurs":[{"ID":50156,"post_author":"0","post_date":"2018-12-04 05:11:55","post_date_gmt":"2018-12-04 04:11:55","post_content":"","post_title":"Bramley","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"bramley","to_ping":"","pinged":"","post_modified":"2018-12-04 05:11:55","post_modified_gmt":"2018-12-04 04:11:55","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/bramley\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":47488,"post_author":"0","post_date":"2018-12-04 04:18:00","post_date_gmt":"2018-12-04 03:18:00","post_content":"","post_title":"Newman","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"newman","to_ping":"","pinged":"","post_modified":"2018-12-04 04:18:00","post_modified_gmt":"2018-12-04 03:18:00","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/newman\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50157,"post_author":"0","post_date":"2018-12-04 05:11:56","post_date_gmt":"2018-12-04 04:11:56","post_content":"","post_title":"Fraser","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"fraser-2","to_ping":"","pinged":"","post_modified":"2018-12-04 05:11:56","post_modified_gmt":"2018-12-04 04:11:56","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/fraser-2\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50158,"post_author":"0","post_date":"2018-12-04 05:11:56","post_date_gmt":"2018-12-04 04:11:56","post_content":"","post_title":"Davuluri","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"davuluri","to_ping":"","pinged":"","post_modified":"2018-12-04 05:11:56","post_modified_gmt":"2018-12-04 04:11:56","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/davuluri\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50159,"post_author":"0","post_date":"2018-12-04 05:11:57","post_date_gmt":"2018-12-04 04:11:57","post_content":"","post_title":"Van Tuinen","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"van-tuinen","to_ping":"","pinged":"","post_modified":"2018-12-04 05:11:57","post_modified_gmt":"2018-12-04 04:11:57","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/van-tuinen\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50160,"post_author":"0","post_date":"2018-12-04 05:11:57","post_date_gmt":"2018-12-04 04:11:57","post_content":"","post_title":"Manfredonia","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"manfredonia","to_ping":"","pinged":"","post_modified":"2018-12-04 05:11:57","post_modified_gmt":"2018-12-04 04:11:57","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/manfredonia\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50161,"post_author":"0","post_date":"2018-12-04 05:11:58","post_date_gmt":"2018-12-04 04:11:58","post_content":"","post_title":"Burgess","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"burgess-3","to_ping":"","pinged":"","post_modified":"2018-12-04 05:11:58","post_modified_gmt":"2018-12-04 04:11:58","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/burgess-3\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50162,"post_author":"0","post_date":"2018-12-04 05:11:59","post_date_gmt":"2018-12-04 04:11:59","post_content":"","post_title":"Brummell","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"brummell","to_ping":"","pinged":"","post_modified":"2018-12-04 05:11:59","post_modified_gmt":"2018-12-04 04:11:59","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/brummell\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":15046,"post_author":"0","post_date":"2018-12-03 19:17:50","post_date_gmt":"2018-12-03 18:17:50","post_content":"","post_title":"Stephen R King","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"stephen-r-king","to_ping":"","pinged":"","post_modified":"2018-12-03 19:17:50","post_modified_gmt":"2018-12-03 18:17:50","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/stephen-r-king\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50163,"post_author":"0","post_date":"2018-12-04 05:11:59","post_date_gmt":"2018-12-04 04:11:59","post_content":"","post_title":"Palys","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"palys","to_ping":"","pinged":"","post_modified":"2018-12-04 05:11:59","post_modified_gmt":"2018-12-04 04:11:59","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/palys\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50164,"post_author":"0","post_date":"2018-12-04 05:12:00","post_date_gmt":"2018-12-04 04:12:00","post_content":"","post_title":"Uhlig","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"uhlig","to_ping":"","pinged":"","post_modified":"2018-12-04 05:12:00","post_modified_gmt":"2018-12-04 04:12:00","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/uhlig\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50165,"post_author":"0","post_date":"2018-12-04 05:12:01","post_date_gmt":"2018-12-04 04:12:01","post_content":"","post_title":"Pennings","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"pennings","to_ping":"","pinged":"","post_modified":"2018-12-04 05:12:01","post_modified_gmt":"2018-12-04 04:12:01","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/pennings\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":50166,"post_author":"0","post_date":"2018-12-04 05:12:01","post_date_gmt":"2018-12-04 04:12:01","post_content":"","post_title":"Bowler","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"bowler","to_ping":"","pinged":"","post_modified":"2018-12-04 05:12:01","post_modified_gmt":"2018-12-04 04:12:01","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/bowler\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"}],"sources":[{"ID":13773,"post_author":"0","post_date":"2018-12-03 19:02:00","post_date_gmt":"2018-12-03 18:02:00","post_content":"","post_title":"NATURE BIOTECHNOLOGY","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"nature-biotechnology","to_ping":"","pinged":"","post_modified":"2018-12-03 19:02:00","post_modified_gmt":"2018-12-03 18:02:00","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/source\/nature-biotechnology\/","menu_order":0,"post_type":"source","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v23.6 (Yoast SEO v23.6) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes - Aprifel<\/title>\n<meta name=\"description\" content=\"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes - Aprifel\" \/>\n<meta name=\"robots\" content=\"noindex, follow\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes\" \/>\n<meta property=\"og:description\" content=\"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes\" \/>\n<meta property=\"og:url\" content=\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/\" \/>\n<meta property=\"og:site_name\" content=\"Aprifel\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/\",\"url\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/\",\"name\":\"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes - Aprifel\",\"isPartOf\":{\"@id\":\"https:\/\/www.aprifel.com\/fr\/#website\"},\"datePublished\":\"2018-12-04T04:12:02+00:00\",\"dateModified\":\"2018-12-04T04:12:02+00:00\",\"description\":\"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes - Aprifel\",\"breadcrumb\":{\"@id\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/#breadcrumb\"},\"inLanguage\":\"fr-FR\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\/\/www.aprifel.com\/fr\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"R\u00e9sum\u00e9s\",\"item\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.aprifel.com\/fr\/#website\",\"url\":\"https:\/\/www.aprifel.com\/fr\/\",\"name\":\"Aprifel\",\"description\":\"Agence pour la Recherche et l\u2019Information en Fruits et L\u00e9gumes\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.aprifel.com\/fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-FR\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes - Aprifel","description":"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes - Aprifel","robots":{"index":"noindex","follow":"follow"},"og_locale":"fr_FR","og_type":"article","og_title":"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes","og_description":"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes","og_url":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/","og_site_name":"Aprifel","twitter_card":"summary_large_image","twitter_misc":{"Dur\u00e9e de lecture estim\u00e9e":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/","url":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/","name":"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes - Aprifel","isPartOf":{"@id":"https:\/\/www.aprifel.com\/fr\/#website"},"datePublished":"2018-12-04T04:12:02+00:00","dateModified":"2018-12-04T04:12:02+00:00","description":"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes - Aprifel","breadcrumb":{"@id":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/#breadcrumb"},"inLanguage":"fr-FR","potentialAction":[{"@type":"ReadAction","target":["https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/fruit-specific-rnai-mediated-suppression-of-det1-enhances\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/www.aprifel.com\/fr\/"},{"@type":"ListItem","position":2,"name":"R\u00e9sum\u00e9s","item":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/"},{"@type":"ListItem","position":3,"name":"Fruit-specific rnai-mediated suppression of det1 enhances carotenoid and flavonoid content in tomatoes"}]},{"@type":"WebSite","@id":"https:\/\/www.aprifel.com\/fr\/#website","url":"https:\/\/www.aprifel.com\/fr\/","name":"Aprifel","description":"Agence pour la Recherche et l\u2019Information en Fruits et L\u00e9gumes","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.aprifel.com\/fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"fr-FR"}]}},"_links":{"self":[{"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/resume\/50167"}],"collection":[{"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/resume"}],"about":[{"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/types\/resume"}],"wp:attachment":[{"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/media?parent=50167"}],"wp:term":[{"taxonomy":"mots_cles","embeddable":true,"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/mots_cles?post=50167"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}