{"id":49938,"date":"2005-11-01T00:00:00","date_gmt":"2018-12-04T04:06:11","guid":{"rendered":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/"},"modified":"2018-12-04T05:06:11","modified_gmt":"2018-12-04T04:06:11","slug":"determination-of-malondialdehyde-mda-by-high-performance","status":"publish","type":"resume","link":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/","title":{"rendered":"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants"},"content":{"rendered":"

A high-performance liquid chromatography (HPLC) method to determine malondialdehyde (MDA) as the 2,4-dinitrophenylhydrazine (DNPH) derivative was applied to biological samples (serum and liver homogenates). Since MDA is considered a presumptive biomarker for lipid peroxidation in live organisms, a model for nutritionally induced oxidative stress (hypercholesterolemic rats) was studied in comparison with normocholesterolemic animals. The effect of diet supplementation with fruits rich in antioxidant polyphenols was assessed. The proposed method showed to be precise and reproducible, as well as sensitive enough to reflect differences in the oxidative status in vivo. A significant decrease of serum and liver MDA concentrations in animals fed diets containing 0.3% of polyphenols from strawberry, cocoa or plum was observed in the normocholesterolemic groups. This reduction was especially noteworthy in the hypercholesterolemic animals, with increased MDA levels indicating enhanced lipid peroxidation in the controls, yet with values parallel to the normocholesterolemic groups in animals fed the polyphenol-rich diets. These results point out the beneficial effects of phenolic antioxidants from fruits in preventing oxidative damage in vivo.<\/p>\n","protected":false},"template":"","mots_cles":[],"class_list":["post-49938","resume","type-resume","status-publish","hentry"],"acf":{"adresse":"Instituto del Frio (CSIC), C\/Jose Antonio Novais 10, Ciudad Universitaria, E-28040-Madrid, Spain.","annee":"2005","mois":"11","numero":"827:1","page":"76-82","auteurs":[{"ID":49932,"post_author":"0","post_date":"2018-12-04 05:06:05","post_date_gmt":"2018-12-04 04:06:05","post_content":"","post_title":"Bravo","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"bravo-2","to_ping":"","pinged":"","post_modified":"2018-12-04 05:06:05","post_modified_gmt":"2018-12-04 04:06:05","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/bravo-2\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":49933,"post_author":"0","post_date":"2018-12-04 05:06:06","post_date_gmt":"2018-12-04 04:06:06","post_content":"","post_title":"Mateos","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"mateos","to_ping":"","pinged":"","post_modified":"2018-12-04 05:06:06","post_modified_gmt":"2018-12-04 04:06:06","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/mateos\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":49934,"post_author":"0","post_date":"2018-12-04 05:06:08","post_date_gmt":"2018-12-04 04:06:08","post_content":"","post_title":"Lecumberri","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"lecumberri","to_ping":"","pinged":"","post_modified":"2018-12-04 05:06:08","post_modified_gmt":"2018-12-04 04:06:08","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/lecumberri\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":49935,"post_author":"0","post_date":"2018-12-04 05:06:09","post_date_gmt":"2018-12-04 04:06:09","post_content":"","post_title":"Ramos","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"ramos-2","to_ping":"","pinged":"","post_modified":"2018-12-04 05:06:09","post_modified_gmt":"2018-12-04 04:06:09","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/ramos-2\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"},{"ID":49936,"post_author":"0","post_date":"2018-12-04 05:06:10","post_date_gmt":"2018-12-04 04:06:10","post_content":"","post_title":"Goya","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"goya","to_ping":"","pinged":"","post_modified":"2018-12-04 05:06:10","post_modified_gmt":"2018-12-04 04:06:10","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/auteur\/goya\/","menu_order":0,"post_type":"auteur_resume","post_mime_type":"","comment_count":"0","filter":"raw"}],"sources":[{"ID":49937,"post_author":"0","post_date":"2018-12-04 05:06:11","post_date_gmt":"2018-12-04 04:06:11","post_content":"","post_title":"JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES","post_excerpt":"","post_status":"publish","comment_status":"closed","ping_status":"closed","post_password":"","post_name":"journal-of-chromatography-b-analytical-technologies-in-the-biomedical-and-life-sciences-2","to_ping":"","pinged":"","post_modified":"2018-12-04 05:06:11","post_modified_gmt":"2018-12-04 04:06:11","post_content_filtered":"","post_parent":0,"guid":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/source\/journal-of-chromatography-b-analytical-technologies-in-the-biomedical-and-life-sciences-2\/","menu_order":0,"post_type":"source","post_mime_type":"","comment_count":"0","filter":"raw"}]},"yoast_head":"\nDetermination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants - Aprifel<\/title>\n<meta name=\"description\" content=\"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants - Aprifel\" \/>\n<meta name=\"robots\" content=\"noindex, follow\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants\" \/>\n<meta property=\"og:description\" content=\"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants\" \/>\n<meta property=\"og:url\" content=\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/\" \/>\n<meta property=\"og:site_name\" content=\"Aprifel\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/\",\"url\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/\",\"name\":\"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants - Aprifel\",\"isPartOf\":{\"@id\":\"https:\/\/www.aprifel.com\/fr\/#website\"},\"datePublished\":\"2018-12-04T04:06:11+00:00\",\"dateModified\":\"2018-12-04T04:06:11+00:00\",\"description\":\"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants - Aprifel\",\"breadcrumb\":{\"@id\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/#breadcrumb\"},\"inLanguage\":\"fr-FR\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\/\/www.aprifel.com\/fr\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"R\u00e9sum\u00e9s\",\"item\":\"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.aprifel.com\/fr\/#website\",\"url\":\"https:\/\/www.aprifel.com\/fr\/\",\"name\":\"Aprifel\",\"description\":\"Agence pour la Recherche et l\u2019Information en Fruits et L\u00e9gumes\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.aprifel.com\/fr\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-FR\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants - Aprifel","description":"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants - Aprifel","robots":{"index":"noindex","follow":"follow"},"og_locale":"fr_FR","og_type":"article","og_title":"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants","og_description":"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants","og_url":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/","og_site_name":"Aprifel","twitter_card":"summary_large_image","twitter_misc":{"Dur\u00e9e de lecture estim\u00e9e":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/","url":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/","name":"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants - Aprifel","isPartOf":{"@id":"https:\/\/www.aprifel.com\/fr\/#website"},"datePublished":"2018-12-04T04:06:11+00:00","dateModified":"2018-12-04T04:06:11+00:00","description":"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants - Aprifel","breadcrumb":{"@id":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/#breadcrumb"},"inLanguage":"fr-FR","potentialAction":[{"@type":"ReadAction","target":["https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/determination-of-malondialdehyde-mda-by-high-performance\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/www.aprifel.com\/fr\/"},{"@type":"ListItem","position":2,"name":"R\u00e9sum\u00e9s","item":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/resume-scientifique\/"},{"@type":"ListItem","position":3,"name":"Determination of malondialdehyde (mda) by high-performance liquid chromatography in serum and liver as a biomarker for oxidative stress application to a rat model for hypercholesterolemia and evaluation of the effect of diets rich in phenolic antioxidants"}]},{"@type":"WebSite","@id":"https:\/\/www.aprifel.com\/fr\/#website","url":"https:\/\/www.aprifel.com\/fr\/","name":"Aprifel","description":"Agence pour la Recherche et l\u2019Information en Fruits et L\u00e9gumes","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.aprifel.com\/fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"fr-FR"}]}},"_links":{"self":[{"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/resume\/49938"}],"collection":[{"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/resume"}],"about":[{"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/types\/resume"}],"wp:attachment":[{"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/media?parent=49938"}],"wp:term":[{"taxonomy":"mots_cles","embeddable":true,"href":"https:\/\/aprifel-pp.mentalworks.biz\/fr\/wp-json\/wp\/v2\/mots_cles?post=49938"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}