Modulation of plasma antioxidant levels, glutathione S-transferase activity and DNA damage in smokers following a single portion of broccoli: a pilot study.

Auteur(s) :
Riso P., Vendrame S., Martini D., Porrini M., ., Bonacina G.
Date :
Fév, 2014
Source(s) :
Journal of the science of food and agriculture., J Sci Food Agric. #94:3 p522-528
Adresse :
Università degli Studi di Milano, DeFENS-Department of Food, Environmental and Nutritional Sciences, via Celoria 2, 20133, Milan, Italy.

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BACKGROUND
Broccoli is a rich source of bioactive compounds (i.e. glucosinolates, carotenoids, vitamin C and folate) that may exert an antioxidant effect and reduce oxidative damage. The objective of this pilot study was to investigate the effect of broccoli consumption on carotenoids, vitamin C and folate absorption, glutathione S-transferase (GST) activity, and oxidatively induced DNA damage in male smokers.

METHODS
Ten healthy subjects consumed a single portion of steamed broccoli (250 g) with cooked pasta. Blood was drawn at baseline and at 3, 6 and 24 h from consumption.

RESULTS
Broccoli significantly (P≤ 0.01) increased plasma level of vitamin C and folate (+35% and 70%, respectively) at 3 h, and β-carotene (+8%) at 6 h. A modulation of GST activity occurred in plasma 6 h after broccoli consumption. A significant (P≤ 0.01) reduction of the levels of H2 O2 -induced DNA damage (-18%) was observed in blood mononuclear cells 24 h after broccoli intake in GSTM1 positive, but not in GSTM1 null subjects.

CONCLUSION
One portion of broccoli increased plasma antioxidant levels, modulated plasma GST activity and improved cell resistance against H2 O2 -induced DNA damage in healthy smokers. These results support the importance of consuming fruit and vegetable regularly.

Source : Pubmed
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