Distribution of dermally absorbed c-14 ddt in the organs of wistar rats

The aim of study was evaluation of the concentration of dermally applied C-14 DDT in the organs of rats, and evaluation of histological and ultrastructural changes after the dermal application of unlabelled DDT standard in the organs of rats, in which the presence of this pesticide was determined by the radio-isotopic method. C-14 DDT of radiochemical purity of 97% was applied in the study. The activity of the preparation per 1 cm(2) of the tail skin was 22.2 KBq – (175.38 mug DDT). The material for the study was taken directly after single exposure and 6 h, 8 h, 10 h, 12 h, 14 h, 18 h and 20 h after exposure. The following organs were taken for the determination of radioactivity of tissues: brain, heart, lung, liver, kidney, skin at the site of exposure. Unlabelled chemically pure, DDT (99.7%) was used to evaluate histological and ultrastructural changes after dermal application. Experimental animals received an oil emulsion of DDT (10 mg/9 cm(2)), applied to the skin of the tail for 4 weeks. The time of exposure was 4 h daily. The animals of the control group were dermally exposed to the emulsion at the same time and under the same conditions. 4 h after dermal exposure it was observed that the preparation applied on the surface of 9 cm(2) in the amount of 1.578 mug was present in the skin in the amount of 1 mug/9 cm(2) (0.11 mug/cm(2)), i.e. 0.063% of C-14 DDT applied dose. Directly after dermal application lasting 4 h the greatest amount of C-14 DDT was noted in the liver – 0.285 mug, i.e. 0.033 mug/g. Histopatological and ultrastructural changes were observed in the liver, kidney, lungs and heart.